Table 3. Normalized summed signal to noise values of the bacillithiol transferases and bacillithiol-related proteins during growth and sporulation.
| Normalized summed signal to noise values | ||
|---|---|---|
| B. subtilis protein | t0 | t3 |
| BstA | 165.1 | 388.9 |
| BstB | 226.4 | 192.2 |
| BstC | 104.5 | 243.6 |
| BstD | 59.2 | 36.7 |
| BstE | 30.4 | 97.4 |
| BstF | NDb | NDb |
| BstG | 180.0 | 122.2 |
| BstH | NDb | NDb |
| BshA | 131.0 | 117.3 |
| BshB1 | 202.3 | 186.5 |
| BshB2 | 196.5 | 165.5 |
| BshC | 338.4 | 220.0 |
| BrxA | 62.6 | 22.4 |
| BrxB | 219.2 | 113.9 |
| YtxJ | 154.9 | 137.4 |
| YpdA | 149.7 | 154.3 |
| CotEa | 113.9 | 334.3 |
| SpoIIQa | 9.9 | 133.5 |
LC-MS/MS analysis using a Thermo Orbitrap Fusion (Thermo Fisher Scientific) was performed on samples collected at the initiation of sporulation (t0), and 3h after the induction of sporulation (t3). Peptides prepared from PY79 was separated by high pH reverse phase HPLC, fractionated and recombined to produce 10 individual injections of tandem mass tag (TMT, isobaric tag analysis). Normalized summed signal to noise values of the reporter ions were used for quantitation.
aSporulation-specific proteins.
bND: not detected.