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. 2017 Jul 17;26(2):83–93. doi: 10.1097/PAI.0000000000000531

FIGURE 2.

FIGURE 2

Western blot and microphotographs of representative examples from PD-L1 clones validation (A and B). PD-L1 MW area from human tonsil cell line, human lung cancer cell lines (H23, H157, H461, H4006, H1171, H193), HEK293, and HEK293-PD-L1 transfected are showing using PD-L1 E1L3N (A), E1J2J (B), SP142 (C), 28-8 (D), 22C3 (E), SP362 (F) 5H1(G) clones, and β-actin (H) by Western blot and immunohistochemistry. Positive membrane staining (brown) and negative PD-L1 staining are shown in reactive tonsil tissue, human placenta, cell pellet from HEK293-PD-L1 nontransfected and transfected cell line. HEK293 indicates human embryonic kidney 293 cell line; MW, molecular weight; PD-L1, programmed cell death ligand 1; transf., transfected.