Figure 2.

Functional NMDARs are present at six weeks of age and do not compromise PCs’ basic electrophysiological properties. A, left, Schematic representation of the recording configuration. Middle, Example traces of CF currents recorded in the presence of the AMPA antagonist NBQX (green) and subsequently of blockers of both AMPA and NMDARs (D-AP5, black) in PCs of both transgenic lines and control littermates. Bold lines are average values; shading indicates individual cell variability. Right, Quantification of NMDA current at the CF-PC synapse in control (Ctrl, black, n = 5) and transgenic animals (Tg1, orange, n = 7; Tg2, red, n = 6). B, Similar to A, with additional example traces of baseline PF-evoked EPSCs before the addition of glutamatergic receptors blockers (blue), and normalized PF-PC NMDA current quantification (Ctrl, n = 9; Tg1, n = 9; Tg2, n = 7). Note that NMDA-mediated currents are only significantly different from controls in Tg2. C, Average firing frequency elicited by somatic current injections from -65 mV in PCs of transgenic (Tg1, orange, n = 5; Tg2, red, n = 12) and control (Ctrl, black, n = 15) mice. The inset illustrates the recording configuration. D, Average amplitude of the EPSCs at the PF-PC synapse to stimuli of increasing intensity for transgenic (Tg1, orange, n = 5; Tg2, red, n = 8) and control (Ctrl, black, n = 18) mice. The inset illustrates the recording configuration. Empty circles represent individual data points, full circles are mean ± SEM; *p < 0.05, **p < 0.01, and absolute p values are indicated in the main text.