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. 2017 Dec 15;96(50):e9160. doi: 10.1097/MD.0000000000009160

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Copyright © 2017 the Author(s). Published by Wolters Kluwer Health, Inc.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. http://creativecommons.org/licenses/by-nc-sa/4.0

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(A) Detection of the e6a2 transcript. M is a Marker X174 DNA Hae III digests. The cDNA major BCR-ABL-positive controls are shown in lanes 2, 4, and 6. The primers to detect b2(e13)a2/b3(e14)a2 and e19/a2, in lanes 1 and 3, respectively, revealed no atypical band. In lane 5, the atypical band was revealed by the primer to detect e1a2. (B) Sequence analysis. An amplified band was formed when analyzed with forward and reverse primers contained in the BCR e1 and ABL a2 regions. The BCR e6 (underlined) region was confirmed, followed by the ABL a2 region.