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. Author manuscript; available in PMC: 2018 Feb 16.
Published in final edited form as: Cell Rep. 2018 Jan 30;22(5):1288–1300. doi: 10.1016/j.celrep.2018.01.001

Figure 3. Egg-Induced IL-1β and IL-17 Production Is Markedly Inhibited in CD209a−/− Cells.

Figure 3

BMDCs were cultured for 24 hr with the indicated numbers of live eggs, curdlan, or LPS plus nigericin (Nig).

(A) Cytokines in supernatants were measured by ELISA.

(B) Cytokine mRNA levels were assessed by qRT-PCR and normalized to Gapdh.

(C) BMDC-T cell co-cultures were stimulated with 80 live eggs, and IL-17A in 72-hr supernatants was measured by ELISA.

(D) BMDCs were cultured for 24 hr with the indicated numbers of live eggs, LPS plus Nig, or Triton X-100 (Tri) for 10 min. Cell survival was measured using calcein AM. The medium was set to 100%.

(E) BL/6 BMDCs were transduced with lentiviruses expressing, CD209a, a carbohydrate binding site mutant of CD209a (R211D/S212G) or RFP control. The cells were stimulated with 80 live eggs, and IL-1β was measured by ELISA. Bars represent the mean ± SD cytokine levels of three biological replicates from one representative experiment of three with similar results.