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. 2018 Feb 5;7:e30842. doi: 10.7554/eLife.30842

Figure 4. Induction of IFN response in human and mouse cells treated with CBL0137.

(A) RT-PCR and immunoblotting of mRNA and lysates from human normal diploid fibroblasts (NDF). ISG56 is a product of the human IFIT3 gene. (B) RT-PCR of mRNA from MEF, wild type or Trp53 (p53) deficient. (C) Immunoblotting of lysate of MEF treated with different doses of CBL0137 (0.03–2 μM) for 24 hr. D and E. Microphotographs of human (D) or mouse (E) tumor cells expressing ISRE-mCherry reporter, treated with different doses of CBL0137 for 48 hr. Poly(I:C) - polyinosinic:polycytidylic acid was used as a positive control for reporter activation at 50 μg/ml.

Figure 4.

Figure 4—figure supplement 1. Comparison of the sensitivity of ISRE reporter assay with detection of the transcript of endogenous ISG, IFIT3 in human tumor cell line HCT116 transduced with lentiviral ISRE-Luc reporter.

Figure 4—figure supplement 1.

Cells were treated for 36 hr with indicated doses of IFNα or CBL0137 and then either used for reporter assay (A) of for RNA isolation and RT-PCR (B).