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. 2018 Feb 5;7:e30842. doi: 10.7554/eLife.30842

Figure 6. Elevated presence of double-stranded RNA in cells and tissues of mice treated with CBL0137.

Immunofluorescent staining of MEF (A) and human HT1080 (B) cells with antibody to dsRNA (red) and Hoeschst (blue). (C) Northern blotting of RNA from MEF (Trp53-/-) treated with either 5-Aza for 72 hr or CBL0137 for 48 hr and hybridized with B1 or Actb probes. (D) Increase in transcripts corresponding to major (GSAT) or minor (SATMIN) satellites or LINE1 in MEF cells or lung tissue of mice treated with either 1 μM or 60 mg/kg of CBL0137 for 24 hr. Bars are relative increase of the transcripts abundance in treated versus untreated samples. p=FDR corrected p-value (see Materials and methods for details).

Figure 6—source data 1. Analysis of expression of repetitive elements in control and CBL0137 treated wild type of Trp53-/- MEF cells of lungs of wild-type mice via RNA-seq.
elife-30842-fig6-data1.xlsx (190.5KB, xlsx)
DOI: 10.7554/eLife.30842.020

Figure 6.

Figure 6—figure supplement 1. INF induction by CBL0137 does not depend on ZBP1.

Figure 6—figure supplement 1.

(A) RT-PCR with primers to ZBP1 of mRNA from Hela cells in which ZBP1 gene was inactivated with CRISP-Cas9 recombinase. Two different gRNA to ZBP1 and one control were used. (B) Fluorescent intensity of ISRE-mCherry reporter in Hela cells control or with inactivated ZBP1 treated with CBL0137 for 48 hr. (C) Immunofluorescent staining of Hela cells treated with CBL0137 for 1 hr with antibody to Z-DNA (red) or Hoechst (blue).