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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Endocrinol. 2018 Jan 25;236(3):151–165. doi: 10.1530/JOE-17-0613

Figure 1.

Figure 1

Direct interaction of CRABP1 with CaMKII. A, Co-immunoprecipitation (IP) of GFP-CaMKII and Flag-CRABP1 in HEK293T cells. B, Co-immunoprecipitation of endogenous CaMKIIδ and CRABP1 in ventricular lysates of healthy normal WT mice. C-D, In vitro interaction assay. Flag-CaMKII captured by M2 beads from TnT synthesized products interacted with His-CRABP1 independent of Ca2+ (C). Reciprocally, His-CRABP1 captured by Ni-NTA beads interacted with Flag-CaMKII independent of Ca2+ (D). TnT refers to synthesis by in vitro transcription and translation.