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. Author manuscript; available in PMC: 2019 Feb 15.
Published in final edited form as: Cancer Res. 2017 Dec 11;78(4):974–984. doi: 10.1158/0008-5472.CAN-17-1686

Figure 1. Doxycycline treatment activates the NFκB pathway in ER positive breast cancer cell lines expressing CA-IKKβ.

Figure 1

A. MCF-7-CA-IKKβ and T47D-CA-IKKβ cells were treated with DOX (1 μg/mL) for 24 or 48 hrs, or with TNFα (10 ng/mL) for 30 min as a positive control. Whole cell extracts were prepared and expression of IKKβ, IKKα, phospho-IκBα (Ser32), IκBα, phospho-p65 (Ser532), p65, and β-actin was detected by Western Blotting. B. MCF-7-CA-IKKβ and T47D-CA-IKKβ cells were treated with DOX for 24 hrs or 48 hrs. Expression of the NFκB target genes, RelB and ICAM1, was assessed by QPCR. (***p<0.001 vs. untreated control).