The percentage of cells immunopositive for CCR7, CD163, and CD206 were averaged within NP, AF, and EP regions for individual IVDs and correlated with Rutges degenerative grade. CCR7+, CD163+ and CD206+ cells all significantly increased with degenerative grade in endplate (EP) regions while only CCR7+ cells significantly increased in NP regions. No changes in percent positivity of any macrophage marker were observed with degenerative grade in AF regions. (N = 12). R = Spearman’s correlation coefficient (95% Confidence Interval).