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. Author manuscript; available in PMC: 2019 Feb 15.
Published in final edited form as: Cancer Res. 2017 Dec 12;78(4):1097–1109. doi: 10.1158/0008-5472.CAN-17-2644

Figure 4. MYB regulates the expression of cell cycle regulatory genes and is co-expressed with CDK6 in different types of leukemia.

Figure 4

A–C, MYB binding by ChIP to regulatory regions of the CDK6 (A), CCND3 (B) or CDKN1A genes (C) in BV173 cells (numbers refer to the position of the forward primer relative to the TSS). As negative controls, ChIPs were performed with a non-targeting antibody (white bars) or with an anti-MYB antibody on lysates from MYB-silenced BV173 cells (grey bars); D–F, plot of the correlation between the mRNA levels (by qPCR) of MYB and CDK6 (D), MYB and CCND3 (E) or MYB and CDKN1A (F) in 24 samples of primary human Ph+ ALL samples; G, plot of the correlation between the expression (by microarray) of MYB and CDK6 in a panel of 226 B-cell ALL of mixed cytogenetics (GSE79533); H, plot of the correlation between the expression (by microarray) of MYB and CDK6 in a panel of 207 Ph-negative childhood, high-risk ALL (GSE11877); I, plot of the correlation between the expression of MYB and CDK6 based on TCGA data on 174 samples of acute myeloid leukemia (AML) of mixed cytogenetics (RNA-seq, median expression).