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. Author manuscript; available in PMC: 2019 Feb 16.
Published in final edited form as: ACS Synth Biol. 2018 Jan 17;7(2):689–695. doi: 10.1021/acssynbio.7b00408

Figure 2. The orthogonality of different OTSs towards UAG or UAA codon.

Figure 2

Lane 1: AcKRS/tRNAPyl; Lane 2: AcKRS/tRNATyr; Lane 3: AcKRS/tRNASep; Lane 4: SepRS-EFSep/tRNASep; Lane 5: SepRS-EFSep/tRNAPyl; Lane 6: SepRS-EFSep/tRNATyr; Lane 7: pTyrRS-EFpY/tRNATyr; Lane 8: pTyrRS-EF-pY/tRNAPyl; Lane 9: pTyrRS-EF-pY/tRNASep. The anticodons of tRNAs were mutated to UUA for UAA codon suppression. For ncAAs, 5 mM AcK, 2 mM Sep, or 10 mM pTyr was added in the medium, respectively. Normalized fluorescence intensities were calculated from fluorescence readings at 12 h divided by absorbance at 600 nm. Mean values and standard deviations were calculated from three replicates.