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. Author manuscript; available in PMC: 2019 Feb 16.
Published in final edited form as: ACS Synth Biol. 2018 Jan 17;7(2):689–695. doi: 10.1021/acssynbio.7b00408

Figure 4. Expression of Sep/AcK-containing MDH.

Figure 4

A) The Coomassie blue-stained SDS-PAGE gel of purified full-length MDH and its variant. The same volumes of elution fractions were loaded on the SDS-PAGE gel. B) Western blotting of purified full-length MDH and its variants. The codons used for positions 140 and 280 were listed. The same amount of proteins were loaded on the gels. C) MALDI-TOF mass spectra of purified full-length MDH (upper) and its variant with AcK incorporated at position 140 and Sep incorporated at position 280 (lower). Full spectra were listed as Figure S5 and S6.