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. Author manuscript; available in PMC: 2019 Feb 16.
Published in final edited form as: ACS Chem Biol. 2017 Dec 20;13(2):357–365. doi: 10.1021/acschembio.7b00855

Figure 2.

Figure 2

Structures of representative type II-C Cas9s and their sgRNAs. (A) The predicted secondary structures of sgRNAs from NmeCas9, GeoCas9, and CjeCas9 are shown. Secondary structures were predicted using mFold. 44 The secondary structure of CjeCas9 as seen in the crystal structure45 is also shown. (B) From left to right: crystal structures of CjeCas9:sgRNA:DNA,45 apo AnaCas9,39 and overlay of CjeCas9 and AnaCas9. The individual domains are colored and are labelled separately. The structures are depicted as cartoons, and were rendered in PyMol using coordinates from pdb entries 4OGE and 5X2H.