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. Author manuscript; available in PMC: 2018 Mar 21.
Published in final edited form as: J Environ Sci Health A Tox Hazard Subst Environ Eng. 2017 Dec 11;53(4):295–309. doi: 10.1080/10934529.2017.1400793

Figure 3.

Figure 3

Activation of human TRPA1-mediated calcium influx in TRPA1-overexpressing HEK-293 reporter cells by cdPM (A) and cdPM extracts (B). Cells were treated for 1 min with constant monitoring of intracellular fluorescence intensity with 25 μL of a 3× suspension of cdPM or corresponding cdPM extracts. Control = medium + DMSO (2% v/v), cdPM suspensions = 2.3 mg/mL equivalent to 180 μg/cm2 and 1.15 mg/mL equivalent to 90 μg/cm2. Data are expressed as mean ± SEM (n=3) and are representative of 3 experiments. N.D. indicates non-detect. *Indicates statistical significance (*p<0.05; **p<0.005) relative to the control. #Indicates difference (p<0.05) relative to other marked groups. The results in Panel A were analyzed by one-way ANOVA, and the results in Panel B were analyzed by two-way ANOVA with with Dunnett's a post-hoc analysis. Solid columns indicate cdPM from the FFB, and hatched columns indicate reference diesel.