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. Author manuscript; available in PMC: 2018 Mar 21.
Published in final edited form as: J Environ Sci Health A Tox Hazard Subst Environ Eng. 2017 Dec 11;53(4):295–309. doi: 10.1080/10934529.2017.1400793

Figure 5.

Figure 5

Effects of the TRPA1 antagonist HC-030031 on CYP1A1 and 1B1 mRNA expression by the cdPM extracts and PAH mixture. Human H441 cells were exposed to cdPM extracts (12.5 and 25 μg/cm2 or 50 and 100 μg/mL) for 24h. Control = medium + DMSO (0.1% v/v). HC-030031 (25μM) was added to the cells at the same time as the extracts. Cells were analyzed for CYP1A1 mRNA transcript levels (A), CYP1B1 mRNA transcript levels (B) and CYP1A1 and 1B1 mRNA transcript levels after PAH treatment (C). Data are expressed as mean ± SEM (n=3). *Indicates statistical significance (*p<0.05; **p<0.005) relative to the control. #Indicates difference (p<0.05) relative to other marked groups. Solid columns indicate cdPM from the FFB, and hatched columns indicate reference diesel.