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. Author manuscript; available in PMC: 2018 Apr 29.
Published in final edited form as: Mucosal Immunol. 2017 Aug 16;11(2):357–368. doi: 10.1038/mi.2017.55

Figure 2. IELs utilize bacterial signals for stimulation of IL-6 secretion.

Figure 2

IELs were harvested from colons of mice and mitogen-stimulated ex vivo. IL-6 secretion into the supernatant was measured by ELISA, and IL-6 from unstimulated IELs was subtracted from that of the mitogen-stimulated IELs from the same mouse. Data are from groups of 3–9 male and female mice aged 8–12 weeks in two independent experiments and shown as the mean ± SEM. Statistical significance was determined by one-way ANOVA with Dunnett’s test. (a) IL-6 secretion from IELs and LPLs isolated from untreated C57Bl/6 mice as well as antibiotic-treated, recolonized, and MyD88−/− mice. *, P<0.05; ****, P<0.0001. ND = not detected; NT = not tested. (b) IEL secretion of IL-6 from germ-free mice gavaged with PBS or monocolonized with bacteria. *,P<0.05