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. Author manuscript; available in PMC: 2018 Apr 29.
Published in final edited form as: Mucosal Immunol. 2017 Aug 16;11(2):357–368. doi: 10.1038/mi.2017.55

Figure 5. IEL produced IL-6 repairs the epithelial barrier and protects from C. rodentium colitis.

Figure 5

(a) IELs from IL-6+/+ or IL-6−/− mice were harvested, magnetically sorted, and transferred into recipient IL-6+/+ or IL-6−/− mice. After one week, intestinal barrier permeability was evaluated by FITC-dextran flux. Two experiments of 2–3 male and female mice per group were performed. Data are the mean serum concentration of dextran ± SEM. ****, P<0.0001 by one-way ANOVA with Tukey’s test. (b) Two days following IEL transfer, 4–7 mice per group were infected with C. rodentium by oral gavage and monitored by daily weights. Mice were euthanized 12 days after infection. Data are the mean percentage of starting weight ± SEM. A two-way repeated-measures ANOVA with Dunnett’s test determined statistical significance. *, P<0.05; **,P<0.01 (c) Methacarn-fixed, paraffin embedded colon tissue from 12-day infected mice in (b) were stained by H&E and evaluated in a blinded fashion for histologic damage as assessed by the number of organized inflammatory aggregates and ulcers along the entire colon. These are shown as the mean ± SEM for each treatment group. *, P<0.05 by Kruskall-Wallis test with Dunn’s multiple comparisons test. (d) Five well-oriented high-powered fields (HPF) per mouse were viewed at 200X and number of crypts counted in each section. Data are the mean crypts/HPF ± SEM. **, P<0.01 by one-way ANOVA with Tukey’s test. (e) Representative histology is shown at 200X. Bar = 50 µm.