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. 2017 Sep 27;25(2):273–277. doi: 10.1016/j.sjbs.2017.09.009

Table 3.

Influence of different cytokinins on shoot regeneration from shoot apex of Ruta graveolens on MS medium, after 8 weeks of culture.

Cytokinins (µM)
% Response Mean no. of shoots Mean shoot length (cm)
BA Kn 2iP
0.0 0.0 0.0 0.0i 0.0h 0.0h
0.5 11.3±1.76fgh 1.8±0.37g 2.2±0.33efg
2.5 15.0±2.51defg 3.4±0.67fg 2.5±0.31defg
5.0 17.3±1.76cdef 6.0±0.54bcd 3.1±0.22bcde
10.0 27.6±1.45a 9.4±0.74a 4.3±0.29a
20.0 22.6±2.67abc 7.4±0.50b 4.0±0.31ab
0.5 8.0±1.15h 2.0±0.31g 1.8±0.33g
2.5 10.6±1.33gh 3.6±0.60efg 2.0±0.35fg
5.0 15.3±2.67defg 5.4±0.74cde 2.8±0.37defg
10.0 22.6±1.76abc 7.6±0.60b 3.5±0.28abcd
20.0 18.0±2.30cde 7.0±0.54bc 3.8±0.20abc
0.5 7.6±1.20h 2.4±0.87g 2.0±0.33fg
2.5 10.3±1.67gh 3.0±0.89g 2.1±0.29fg
5.0 12.0±1.15efgh 4.6±0.67def 2.5±0.27efg
10.0 25.0±2.88ab 7.4±0.50b 2.9±0.38cdef
20.0 21.0±2.08bcd 6.4±0.50bcd 2.7±0.34defg

Data shows mean ± standard error of 20 replicates per treatment in three repeated experiments. Means followed by the same alphabets are not significantly different (P = 0.05) using Duncan’s multiple range test.