Table 2.
Comet assay for fish species, site and type interaction analyzed for Labeo rohita. Fish species and site interaction (mean ± SE).
| Sites | Comet assay components |
||||
|---|---|---|---|---|---|
| Head diameter (px) | Tail length (px) | DNA in tail (%) | Tail moment | Olive moment | |
| Site R1 | 63.33 ± 2.20a | 16.66 ± 1.65a | 19.14 ± 1.38a | 6.46 ± 0.79a | 5.31 ± 0.51a |
| Site R2 | 83.59 ± 3.38bc | 16.20 ± 1.63ab | 16.38 ± 1.26b | 4.72 ± 0.69b | 5.14 ± 0.52ac |
| Site R3 | 66.28 ± 2.13a | 19.07 ± 1.81c | 19.95 ± 1.33a | 7.14 ± 1.08c | 6.01 ± 0.49b |
| Mean | 71.07 ± 1.56C | 17.31 ± 0.98C | 18.49 ± 0.77A | 6.11 ± 0.50B | 5.49 ± 0.29B |
| Fish species and type interaction (mean ± SE) | |||||
| Control +ve | 84.03 ± 4.31d | 14.67 ± 1.86a | 15.98 ± 1.42bc | 4.50 ± 0.77cd | 4.96 ± 0.59c |
| Farmed | 58.88 ± 1.24b | 2.19 ± 0.43c | 4.61 ± 0.63d | 0.35 ± 0.11d | 0.88 ± 0.14d |
| Polluted | 75.01 ± 2.62c | 31.83 ± 1.84b | 31.77 ± 1.45a | 11.94 ± 1.04b | 9.19 ± 0.59b |
| Upstream | 66.34 ± 3.15a | 20.55 ± 2.31a | 21.60 ± 1.53b | 7.63 ± 1.38bc | 6.92 ± 0.63bc |
| Fish species, site and type interaction (mean ± SE) | |||||
| R1. +ve | 59.96 ± 4.32cd | 16.70 ± 3.73cd | 16.58 ± 2.32c–f | 5.64 ± 1.68fg | 4.34 ± 0.92ef |
| R1. F | 67.06 ± 1.61e–g | 3.42 ± 0.99ab | 4.61 ± 1.15d | 0.54 ± 0.29bc | 1.02 ± 0.30e |
| R1. P | 88.10 ± 4.85bc | 39.92 ± 3.31d–f | 42.21 ± 2.06ab | 17.71 ± 1.79a | 13.58 ± 1.06ab |
| R1. U | 38.20 ± 2.86ef | 6.58 ± 1.26ab | 13.18 ± 1.96d | 1.96 ± 0.59cd | 2.28 ± 0.38e–g |
| R2. +ve | 122.32 ± 8.53b–e | 14.08 ± 3.48de | 13.31 ± 2.40d–f | 2.54 ± 0.99g | 5.07 ± 1.13f |
| R2. F | 63.10 ± 2.40e | 1.46 ± 0.51ab | 4.77 ± 0.97de | 0.20 ± 0.11cd | 0.92 ± 0.21de |
| R2. P | 59.88 ± 3.81a–c | 27.12 ± 3.16g | 31.26 ± 2.41a–c | 10.30 ± 1.79a–c | 8.10 ± 1.04bc |
| R2. U | 89.06 ± 6.46f | 22.14 ± 3.62a–c | 16.17 ± 2.45d | 5.83 ± 1.54de | 6.48 ± 1.18fg |
| R3. +ve | 69.80 ± 5.67bc | 13.22 ± 2.33d | 18.05 ± 2.67d–f | 5.32 ± 1.21ef | 5.47 ± 1.02d–f |
| R3. F | 46.48 ± 0.85a | 1.68 ± 0.60ab | 4.46 ± 1.14de | 0.33 ± 0.13b | 0.70 ± 0.19c |
| R3. P | 77.06 ± 4.06b–d | 28.44 ± 2.82e–g | 21.84 ± 2.21bcd | 7.81 ± 1.57ab | 5.88 ± 0.60de |
| R3. U | 71.76 ± 3.61ae | 32.94 ± 5.17bc | 35.45 ± 2.36df | 15.10 ± 3.57a–d | 11.99 ± 1.06fg |
R1–R3; polluted experimental sites along the River Chenab, Fish Types (P; polluted, F; farmed, +ve; positive control, U; upstream). Means sharing similar letter in a column are statistically non-significant (P > .05). Small letters represent comparison among interaction means and capital letters are used for overall mean.