Fig. 4.

MiR-128 deletion is associated with activation of cell cycle-related genes. a The predicted conserved target site of miR-128 in the 3′UTR of Suz12 from different species. b, c Western blot analysis of SUZ12 expression in mouse hearts at P1, P7, and P28 (n = 5). d, e Western blot analysis of SUZ12 expression in neonatal CMs treated with either vehicle (Ctrl), miR-128 mimic (miR-128) or miR-128 inhibitor (Anti-miR-128) (n = 3). f Luciferase reporter assay for wild-type (WT) and mutant Suz12 3′UTR (Mut) in cells treated with either vehicle (Ctrl) or miR-128 mimic (miR-128) (n = 3). g Western blot assay for cell cycle-related protein expression in control (miR-128^fl/fl), and miR-128^−/− (Nkx2.5^Cre; miR-128^fl/fl) hearts at P7 (n = 5). h Quantification data of p27 mRNA levels in Ctrl and miR-128^−/− hearts by qPCR (n = 5). i Comparison of SUZ12, EZH2, and H3K27me3 enrichment on the p27 promoter by ChIP-qPCR (n = 5). Statistical significance was calculated using ANOVA in c, e and Student’s t-test in f, h, and i. Data are represented as means ± SEM. *P < 0.05. NS designates not significant