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. 2018 Feb 12;9:194. doi: 10.3389/fimmu.2018.00194

Figure 2.

Figure 2

Bm-L3 infection differentially modulates the functions of host MΦs. (A) Histograms represent expression of maturation and costimulatory markers viz. MHC II, CD80, and CD86 on FACS-sorted splenic MΦs from uninfected control and Bm-L3 infected mice at day 3 and day 7 p.i. as determined by flow cytometry. Values in bracket are mean fluorescence intensity (MFI) values at given time points. (B,C) Antigen uptake (phagocytosis) and antigen-processing capacity of FACS-sorted splenic MΦs from uninfected control and Bm-L3-infected mice at day 3 and day 7 postinfection is shown. Values represent % phagocytic cell or MFI values as determined by FITC-Dextran uptake or DQ-ova-albumin cleavage, respectively. (D) Mitochondrial activity, as a measure of T cell proliferation was assessed by XTT assay. Figure represents OD values of FACS-sorted splenic MΦs cocultured with naïve CD4+ T cells at 450 nm at indicated time points. (E) Concentration of different lipid intermediates present in the culture supernatant of FACS-sorted splenic MΦs is shown. All values represent mean ± SD values from three independent experiments with at least 3–4 animals/group. p-value of ≤0.05, ≤0.01, and ≤0.001 was considered significant, highly significant, and very highly significant and marked with *, **, and ***, respectively.