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. 2018 Feb 12;12:5. doi: 10.3389/fnint.2018.00005

Figure 1.

Figure 1

Histological characterization of eYFP+ neurons in Trpv1Cre;Ai32 and Scn10aCre;Ai32 mice. Histological characterization of L6-S1 dorsal root ganglia (DRG) from Trpv1Cre;Ai32 (A–C) and Scn10aCre;Ai32 (E–G) mice confirms eYFP reporter expression in bladder projecting CTβ+ DRG neurons. Arrows indicate neurons that co-label for CTβ and eYFP. Voltage clamp recordings from isolated Trpv1Cre;Ai32 (D) and Scn10aCre;Ai32 (H) ChR2+ DRG neurons show 1 s blue light stimulation resulted in inward current. (I) In Trpv1Cre;Ai32 mice, eYFP+ terminals were observed in the most superficial layers of the dorsal horn (DH; laminae I-II), and to a lesser degree in lamina X (dorsal commissure; dcm) with sparse projections to the sacral parasympathetic nucleus. dc, dorsal columns. (J) In Scn10aCre;Ai32 mice, dense eYFP+ afferent terminal distribution is observed in laminae I-V and X, with very few terminals in the sacral parasympathetic nucleus. (K,L) In bladder, tissue, eYFP+ fibers were seen in both mouse lines branching from large nerves at the base of the bladder and the outer muscular layers, coursing toward the lumen. Fibers often terminated in the urothelium in Trpv1Cre;Ai32 mice. In Scn10aCre;Ai32 mice, the parasympathetic postganglionic neurons (pg) at the base of the bladder also expressed eYFP (arrowheads). (M,N) Cumulative distributions of cell area for eYFP+ CTβ-labeled neurons in Trpv1Cre;Ai32 and Scn10aCre;Ai32 mice did not statistically differ (Kilmogorov-Smirnov test, P = 0.97, n = 107 for Trpv1Cre;Ai32 and n = 100 for Scn10aCre;Ai32). Scale bars represent 100 μm for (A–G) and 50 μm for (I–L).