Fig. 1.

Small FLIPPER-bodies for single-step CLEM labeling. a Left: traditional indirect antibody labeling conjugated with QD, represented by black dot. Right: a single-labeling step with FLIPPER-body. Black circle represents DAB conversion. Black triangle: antigen. Bar 2 nm.

Adapted from (Beghein and Gettemans 2017; Giepmans et al. 2006; Martell et al. 2012). b Boxes representing the modules of the anti-GFP and anti-EGFR FLIPPER-bodies. The boxes are shown in proportion to each other. Signal peptide (#), His6-tag (*), thrombin cleavage site (^), mCherry (mCh), dTomato (dTom), horseradish peroxidase (HRP), ascorbate peroxidase 2 (APEX2), anti-GFP nanobody (anti-GFP), anti-EGFR nanobody (anti-EGFR)