Fig. 3.

FLIPPER-body targets specific overexpressed EGFR. HEK293T expressing EGFR-GFP. a Detection of EGFR by GFP fluorescence, as well as by indirect labeling of EGFR with secondary antibodies conjugated to QD655. Note that QD655 are detected at the periphery of the cells, but not at cell–cell contact sites. b Detection of EGFR using FLIPPER-body (cartoon right). The FLIPPER-body labels uniquely the EGFR-GFP positive cells, including at the cell–cell contact sites. DIC differential interference contrast, GFP GFP fluorescence, QD655 QD655 fluorescence, dTomato FLIPPER-body, merged GFP and label, EM ultrathin EM section. Bars LM 10 µm, EM 5 µm, EM zoom in 0.5 µm. High-resolution EM images are available via http://www.nanotomy.org