Fig. 4.

FLIPPER-body reveals localization of endogenous EGFR. a EGFR on A431 cell was labeled using anti-EGFR and secondary IgG conjugated with QD655. Note that the QD655 labeling is mainly restricted to the periphery of the cell clusters. b EGFR is labeled with FLIPPER-body shown at the top. The cell periphery as well as the cell–cell contact sites are labeled with fluorescence and black staining in EM. DIC differential interference contrast, QD655 QD655 fluorescence, dTomato FLIPPER-body. Bars LM 20 µm, EM 10 µm and EM zoom in 200 nm. High-resolution large-scale EM images are available via http://nanotomy.org