Fig. 5.

Correlated microscopy of endogenous HER2. SkBr3 cells labeled and processed for CLEM. a HER2 detection with anti-HER2 FLIPPER-body with dTomato fluorescence. In EM, a black substrate is surrounding the cells. b Overlap of the fluorescence and EM image resulting in the CLEM image. c Zoom in EM images shows the labeling at the cells periphery and cell–cell contact sites (green zoom in). It is also noted that there is a heterogeneous distribution of the HER2 labeling (blue zoom in). DIC differential interference contrast, LM dTomato fluorescence, EM ultrathin EM section. Bars LM 10 µm, EM 10 µm and EM zoom in 500 nm. High-resolution EM images are available via http://www.nanotomy.org