Fig. 1.

The loss of Arl13b reduces Shh pathway output in SmoA1-GFP MEFs. (A) qRT-PCR for Shh targets Gli1 and Ptch1 shows pathway activation is lowered when Arl13b is deleted in the presence of SmoA1. All data are mean ± SEM of three biological replicates; ⁺⁺P < 0.005 and ⁺⁺⁺P < 0.0005 between genotypes within a given treatment; ^••P < 0.005 and ^•••P < 0.0005 compared with untreated within a given genotype. (B) Western analysis of Gli processing in untreated and Shh-stimulated SmoA1-GFP MEFs with and without Arl13b. (C) Bar graphs show quantification of B. Data are mean ± SEM of at least three biological replicates; ⁺⁺P < 0.005 represents genotype significance, ^•P < 0.05 represents treatment significance; S.Q., starting quantity.