Fig. 5.
Effects of P expression on long-term spatial memory in the Morris water maze. (A) Schematic representation of the setup and experimental timeline. (B) Mean distance traveled to find the hidden platform during training (one-way ANOVA with repeated measures *P < 0.001). (C and D) Effects of P expression on spatial memory during the 24 h posttraining probe test. (C) Time (seconds) spent in each quadrant of the pool, showing that all mice spent more time in the target quadrant compared with the three others (***P < 0.001 by one-way ANOVA intragroup analyses). Comparison of time spent in the target quadrant between PWT mice and GFP and PAASS animals. One-way ANOVA, #P < 0.05. (D) Spatial search precision: analysis of the number of annuli crossings between groups of mice. *P < 0.05 for PWT and ***P < 0.001 for GFP and PAASS, by one-way ANOVA intragroup analyses. (P > 0.65 and P > 0.07 for, respectively, target vs. east and target vs. west, by post hoc Fisher’s least significant difference test). Data are expressed as means ± SEM (GFP n = 20; PWT n = 25; PAASS n = 23).