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The end-to-end distance of the rU40 long ssRNA simulated with AMBER ff14 and TIP3P (A) or the revised parameters and TIP4P-D (B) under 0.05 M NaCl at 300 K. See SI Appendix, Figs. S7 and S8 for other NaCl concentrations. (C) The 〈RFRET〉 values of rU40 under different NaCl concentrations. The experimental values were reported in ref. 36. See Methods for the procedure of calculating the simulated 〈RFRET〉 values.
