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. 2018 Jan 30;115(7):E1578–E1587. doi: 10.1073/pnas.1714645115

Fig. 4.

Fig. 4.

Inhibition of the P cycle abrogates metabolite-mediated killing. (A) NAD+/NADH and PMF of EIB202. Cells were grown in LB medium and then incubated in M9 medium plus acetate (10 mM) with and without glutamate (2.5 mM) in the presence or absence of bromopyruvate (2.5 mM), furfural (10 mM), or malonate (20 mM), as indicated. (B) Percent survival of EIB202. Cells were grown in LB medium and then incubated in M9 medium plus acetate (10 mM) in the presence or absence of oxaloacetate (10 mM) and PEP (1.25 mM), pyruvate (10 mM), or acetate (10 mM) with or without furfural (10 mM), bromopyruvate (2.5 mM), or malonate (20 mM). (C) Percent survival of EIB202. Cells were grown in LB medium and then incubated in M9 medium plus acetate (10 mM) in the presence or absence of alanine (40 mM) or glucose (10 mM) and effect of inhibitors furfural (10 mM) or malonate (20 mM). Results are displayed as mean ± SEM, and three biological repeats were carried out. Statistically significant differences are indicated by asterisk (*P < 0.05, **P < 0.01) and were determined by Student’s t test.