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. 2018 Jan 30;115(7):E1578–E1587. doi: 10.1073/pnas.1714645115

Fig. 6.

Fig. 6.

Effect of exogenous glutamate on the P cycle and the TCA cycle in E. coli. (A) Percent survival of E. coli K12 and selected P cycle mutants. Cells were grown in LB medium and then incubated in M9 medium with acetate (10 mM) in the presence or absence of glutamate (2.5 mM) plus gentamicin (1.2 μg/mL). (B and C) NAD+/NADH (Left) and PMF (Right) of E. coli K 12 (B) and selected P cycle mutants (C). Cells were grown in LB medium and then incubated in M9 medium plus acetate (10 mM) in the presence or absence of glutamate (2.5 mM). (D) Effect of pck, aceE, and gltA deficiency on abundance of the indicated metabolites. Cells grown in LB medium and then incubated in M9 medium plus acetate (10 mM) in the presence or absence of glutamate (2.5 mM). Results (A–D) are mean ± SEM of three biological repeats. Statistically significant values are indicated with an asterisk (*P < 0.05, **P < 0.01) and were determined by Student’s t test.