Skip to main content
. 2018 Jan 29;115(7):E1366–E1373. doi: 10.1073/pnas.1714790115

Fig. 7.

Fig. 7.

Metabolic incorporation of AzC and reversible inhibition of DNA synthesis in the retinal stem cell niche of zebrafish larvae. (A) Staining patterns of AzC and AmdU after 24-h treatment of 3-d-old fish with 10 mM AmdU or 5 mM AzC, fixation, and staining with SiR alkyne. (B) Lack of overlap between nucleolin immunofluorescent staining (arrows) and AzC after 24-h treatment of 3-d-old zebrafish with 5 mM AzC. (C) Overlap between AzC and PCNA staining after 24-h treatment of 3-d-old zebrafish with 5 mM AzC. (D) Inhibition of DNA synthesis by addition of AzC or ara-C. Three-day-old fish were incubated with 5 mM AzC, ara-C, or AmdU for 3 h, followed by coincubation with 1 mM F-ara-EdU for 21 h. F-ara-EdU was visualized using Alexa Fluor 488 azide. (E) Resumption of DNA synthesis after removal of AzC or ara-C. Following a 24-h pulse of 5 mM AzC, ara-C, or AmdU, the fish were moved into fresh water containing 1 mM F-ara-EdU for a 60-h recovery period before fixation and staining. (F) Movement of AzC-labeled DNA out of the retinal stem cell niche according to an AzC pulse, F-ara-EdU chase experiment as described in E. See SI Appendix, Figs. S29–S37 for the corresponding images of the liver, intestine, and brain.