Fig. 8.

OLT1177 reduces IL-1β release in peripheral blood mononuclear cells (PBMCs) from human subjects with NLRP3 mutations. Mean levels of IL-1β in the supernatants of PBMCs from two patients affected by CAPS. Patient A (A) represents a severe case of neonatal onset multisystem inflammatory disease (NOMID) with mutation E567K, and patient B (B) represents a case of Muckle–Wells syndrome (MWS) with mutation E525K. Cells were stimulated with LPS (100 ng/mL) for 18 h in the presence of vehicle (PBS) or OLT1177 (1 µM) (OLT). Vehicle and OLT1177 were added 30 min after LPS.