Fig. 4.

KRAS, NOTCH1, and MYC programs drive tumor development in Cic adult knockout mice. (A) Chromatogram traces showing that two of the Cic-null tumors harbor mutations in Notch1. The sequences of wild-type and mutant alleles are indicated. (B) Heatmap showing clustering of differentially expressed genes (DEGs) (FDR < 0.05, Log₂ fold change > 3) from the tumor RNA-seq analysis. (C) Gene set enrichment analysis (GSEA) reveals signatures of activation of KRAS, NOTCH1, MYC, and mTORC1 signaling in Cic knockout tumors.