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. 2018 Jan 29;115(7):1617–1622. doi: 10.1073/pnas.1716801115

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Fig. 2. — Age-dependent labeling of DCVs expressing ANF tagged with mK-GO in synaptic boutons. (A) Green (young) and red (old) images are shown side by side for control (Con) boutons, Myopic RNAi knockdown (RNAi), and overexpressing (OE) boutons. The contrast was adjusted to normalize red images. (Scale bar, 2 μm.) (B) Quantification of green-to-red fluorescence (F) ratio in Con (four animals), RNAi (no. 34085, three animals), and OE (four animals) NMJs. Numbers on the bars represent the number of boutons analyzed. ****P < 0.0001, ANOVA with Dunnett’s posttest.