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Thermodynamics of αVβ6 fragment binding to pro-TGF-β1 measured with ITC in 1 mM Mg2+ and 1 mM Ca2+. (A–D) Data are shown for the indicated constructs. (E) Affinity and thermodynamics. Values are shown ± fitting error from Origin 7. *Affinity was too high to be measured by ITC; KD was taken from SPR and used to calculate TΔS.
