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Reduced histone H2A ubiquitylation in patient-derived lymphoblasts. Whole-cell lysates from lymphoblasts of the patient (P) and three unrelated female controls (C1, C2, C3) and from HEK293T cells were analyzed by immunoblotting. Upper are representative immunoblots; Lower are means ± SD of three independent experiments. (A) Levels of ubiquitylated histone H2A (H2A-Ub1) relative to H2A. (B) Levels of RING1 relative to HPRT. (C) Levels of RING2 relative to HPRT.
