Fig. 3.

Wnt3a induces and PKF115–584 inhibits migration and typical features of EMT of melanoma cells in vitro. a Scratch assay of human SKMEL28 (upper row) and A375 (lower row) melanoma cells showing increased migration upon stimulation with Wnt3a conditioned medium (Wnt3a CM) and decreased migration upon treatment with PKF115–584) when compared to untreated and 3T3-medium conditioned cells (3T3-CM). b Upper row/ Aggregation: Aggregates were generated from SKMEL28 cells (untreated, or stimulated by Wnt3a or 0.5 μM PKF115–584 during aggregation), photographed and measured after 24 h. Wnt3a decreased and PKF115–585 increased the aggregate size (*: p < 0.05; ***: p < 0.001, One way ANOVA). Depicted: aggregate size in μm²±SD. Lower row/ Outgrowth: The SKMEL28 aggregates were re-incubated for 24 h in 6-well-plates and photographed. Around each aggregate a single layered outgrowth of emigrating cells had formed. The areas of aggregates and cellular outgrowths were measured and the migration capacity (cell migration index) determined by dividing the area of the outgrowth by the area of the aggregate. Wnt3a increased and PKF115–584 decreased the cell migration index (***: p < 0.001, One way ANOVA). Depicted: cell migration index±SD