Table 1.
Primer sequences, product size, MgCl2 concentration and annealing temperatures used in RT-PCR analyses
| Gene | Primer sequence (5′-3′) | Product size (bp) | MgCl2 (mM) | Annealing temperature (°C) |
|---|---|---|---|---|
| CA3 | F: TGAAGCAGCGCGATGGGAT R: GTCAGAGCTCACGGTCATGGGC |
260 | 2 | 66 |
| FHL1 | F: CCGCTTCTGGCATGACACCT R: ACGGTCCCCTTGTACTCCACG |
189 | 2 | 66 |
| ANXA6 | F: -CCGGCACAGATGAAAAGGCTC R: TTCTCCTCCCTCCTCACGATGC |
191 | 2 | 66 |
| WDR26 | F: TGCCAATTGCGGAGCTGACA R: CGTCTGCTCCAAATTCACCATCAA |
196 | 2 | 66 |
| HMGN4 | F: CCTTCCCTCGCCTTCCTGTTCC R: TGTCCTCCTCACGCTGTTCCTGG |
182 | 1 | 66 |
| C9orf64 | F: AGGCTCTTTTCTCAACTGCGTCCGT R: AGCAGCCATCTCCTTTTCCTTCCA |
191 | 2 | 66 |
| FSTL1 | F: CCCAGACCCAGACAGAGGAGGAG R: ACTGGTGATTTGGCGACTGTAGCA |
203 | 2 | 66 |
| CCN1 | F: GCAATTCAGAGGATCCATG R: GGTGTGCTTGAGGGGACGGTAG |
220 | 3 | 55 |
| SAR1B | F: ACCACGAAAGGCTGTTAGAGTCAAAA R: AACCAAACATCTCTCGCAACCTCTC |
146 | 2 | 66 |
| NFE2L1 | F: ACGGAACCTGCTAGTGGATGGAGA R: CTGTTATGCTGGAAATGTCTGCTGGA |
167 | 1 | 70 |