FIG. 9.

Histological analysis of orthotopic de novo pulp regeneration in mini-swine. (A–D) Radiographs of a female Yucatan mini-swine mandibular right third premolar receiving pulpectomy, canal enlargement and allogeneic cell transplantation. (A) Pre-op; (B) during pulpectomy checking the working lengths (∼20 mm); (C) after injection of DPSCs (passage 3)/HyA into the canals, the tooth was sealed with MTA and bonding composite; (D) ∼6 weeks later at sacrifice showing tooth block (Left, buccal-lingual view; Right, mesial-distal view). (E) Longitudinal view of the mesial root showing root canal space filled with rP tissue. (F) Closer view of the coronal area of the sample showing regenerated dentin-like (rD) mineral formation on the existing dentin walls (D) and odontoblast-like cells (Od) (black arrow). (G–I) Higher magnification views of rD, DB, rP and Od. Note: *Sign indicates the area of rP is loose or lacking normal pulp-like tissue caused by the remaining HyA. **denser soft tissue similar to normal pulp.