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. 2018 Feb 17;18:11. doi: 10.1186/s12896-018-0416-3

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Schematic map of the vectors used for Agrobacterium-mediated transformation. Only the portion between the LB and RB (T-DNA) is shown. All vectors contain the Paro gene, conferring resistance to paromomycin under the control of the strong HSP70/RBCS2 hybrid promoter (P1) and RBCS2 terminator (T1). pAgroR (a) contains an expression cassette containing the PSAD promoter (P2) and terminator (T2) sequences. In pAgroLucR (b) the Luc coding sequence is cloned in the expression cassette. In pAgroLucL (c) the Paro and Luc cassettes have an inverted orientation with respect to pAgroLucR. For further details see Material and Methods