Table 2.
Comparison of electroporation and Agrobacterium-mediated transformation. Transformation efficiencies are expressed as the number of paromomycin resistant colonies/108 cells transformed. Presence of Paro and Luc transgenes was tested by PCR. Transformants containing the Luc transgene were further analyzed for Luc enzymatic activity (last column). Results of two independent experiments are reported. At least 96 independent transformants were analyzed for each experiment
| Transformation method | Plasmid | Transformants/108 cells | Paro-positive transformants (PCR) | Luc-positive transformants (PCR) | Transformants exhibiting Luc activitya |
|---|---|---|---|---|---|
| Electroporation | pAgroLucR | 120 | 100% | 26% | 15% |
| 250 | 100% | 33% | 20% | ||
| Agrobacterium (C58C1) | pAgroLucR | 4 | 97% | 16% | 13% |
| 52 | 65% | 13% | 11% | ||
| Agrobacterium (C58C1) | pAgroLucL | 31 | 93% | 23% | 20% |
| 12 | 49% | 13% | 10% |
aLuc activity > 3-fold that of the untransformed control