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. 2018 Feb 17;17:25. doi: 10.1186/s12934-018-0874-2

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — a Paulomycin gene cluster knockout strategy. b PCR confirmation of 15 kb deletion from the paulomycin gene cluster in different backgrounds. M, 1 kb ladder. Solid black arrows represent primers used for PCR screening and sizes of the PCR products are indicated. Confirmation of upregulation of paulomycin gene cluster c in triple mutant S. albus ΔpfkΔwblA+ermE*crp_SC versus S. albus ΔpfkΔwblAΔplm+ermE*crp_SC by LC–MS profiling of culture extracts produced following 6 days of growth in R5A media and d antimicrobial assay of culture extracts derived from S. albus and engineered strains grown for 4 days in R5A media against Bacillus cereus. Minimum inhibition concentration for both S. albus+ermE*crp_SC and ΔwblA derived extracts are reported to be 7.8 µg/ml