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. 2018 Feb 16;8:3192. doi: 10.1038/s41598-018-21107-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scanning electron microscopy of spermatozoa. (a–c) Spermatozoon of a negative control GFP(RNAi) worm. (a) Overview of the complete cell. Note the curved view of the shaft. (b) Detail of the brush, consisting out of separate extensions. (c) Detail of the notch region of the cell. (d–f) Spermatozoon of a Mlig-sperm1(RNAi) worm. (d) Overview of the complete cell. Note the rigidity of the shaft and the contortion at the notch site. (e) Detail of the brush. Compared to the negative control the brush looks more flattened with the base of the extensions being more packed together. (f) Detail of the notch region clearly showing the contortion. Scale bar are 10 µm (a,d) and 2 µm (b,c,e,f).