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. 2018 Feb 16;8:3192. doi: 10.1038/s41598-018-21107-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ultrastructure of spermatids and spermatozoa. (a,b) Overview of early and late spermatids and spermatozoa in the testes of a negative control GFP(RNAi) worm (a) and a Mlig-sperm1(RNAi) worm (b). In both images, several longitudinal and cross sections of nuclei of the spermatozoa can be observed as black structures. (c,d) Detail of longitudinal sections of spermatozoa nuclei of a GFP(RNAi) worm. The chromatin of the nucleus is condensed into discrete bodies. (e) Detail of a cross section of spermatozoa nuclei of a GFP(RNAi) worm. (f,g) Detail of longitudinal sections of spermatozoa nuclei of a Mlig-sperm1(RNAi) worm. Compared to the negative control, the chromatin of the nuclei looks fragmented and condensation into discrete bodies is less visible. (h) Detail of a cross section of spermatozoa nuclei of a Mlig-sperm1(RNAi) worm. Compared to the negative control, the chromatin of the nuclei is more fragmented. Scale bars are 5 µm (a,b) and 500 nm (c–h).