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. 2018 Feb 16;8:3192. doi: 10.1038/s41598-018-21107-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fertility of healthy control and Mlig-sperm1 knockdown M. lignano animals. (a) Fertility of 4 groups of Mlig-sperm1(RNAi) worms and 4 groups of adult negative control GFP(RNAi) worms. The RNAi treatment started with adult animals and was continued for a total of 3 weeks. Data points are indicated by black dots, the black line represents the median, and the cross indicates the mean. The data points represent the number of hatchlings produced by each group of five worms within three weeks. The star represents a significant difference with a p-value < 0.05. (b) Fertility of 6 groups of Mlig-sperm1(RNAi) worms and 6 groups of negative control GFP(RNAi) worms. The RNAi treatment started with one-day old hatchlings and was continued for a total of 5 weeks. The number of hatchlings produced by each group of 5 worms within this period is visualized as a boxplot combined with a beeswarm of the data points. Data points are indicated by black dots, the black line represents the median, and the cross indicates the mean. The double star represents a significant difference with a p-value < 0.01.