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. 2018 Feb 16;8:3177. doi: 10.1038/s41598-018-21534-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) The level of cAMP-CRP synthesized in the cell from pET_CRP after IPTG induction. (b) cAMP-CRP interacted with the yihU/yihT, yihV/yihW, and yihV/yihU intergenic regions, with most efficient binding being registered for yihV/W. The protein:DNA ratio is indicated above the lanes. Control lane for the yihV/yihW intergenic region was run on a separate gel, as well as the sample with 1:1 protein:DNA ratio (this ratio gave no relevant binding and the corresponding lanes were deleted from the final figure). (c) Binding of purified CRP to the studied regions with/without cAMP. The protein:DNA ratio and the presence of heparin in the sample is indicated above the lanes. (d) Multiple alignment of the yihV/W intergenic region in several bacteria containing orthologs for these genes revealed high conservation of the mapped yihW promoter and the CRP binding motifs.