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. 2018 Feb 16;8:3167. doi: 10.1038/s41598-018-21548-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Myocardial histological changes after TAC with the DLC technique. Macrophotographies of LV sections stained with Masson trichrome of (a) a control mouse, and mice subjected to (b) TAC and (c) TAC and de-TAC. Light microscopic studies revealed (d) interstitial and (e) perivascular fibrosis, and allowed (f) morphometric assessment of the cardiomyocytes. (g) Cardiomyocyte diameter increased after TAC in the endocardial stratum of the posterior wall. These changes regressed after release of the aortic constriction. (h) The dispersion of myocyte diameter values after TAC was significantly greater with Rockman’s technique than with DLC (Bartlett’s test). Comparisons in G with one way ANOVA. **p < 0.01; ***p < 0.001. DLC: Double loop-clip.