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. 2018 Feb 16;8:3176. doi: 10.1038/s41598-018-21621-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histopathological effect of TUDCA by Luxol Fast Blue/cresyl violet-staining. Lesion epicenter histopathology (luxol fast blue/cresyl violet-staining staining): (A) Spinal cord tissue on 1 day after spinal cord injury. (B) Spinal cord tissue on 3 day after the injury shows more remaining myelin fiber and observes nerve cells in the TUDCA group. (C) Spinal cord tissues on 7 day after the injury are similar in the TUDCA and sham groups (Scale bar = 200 µm). Results are the mean ± SD of triplicate experiments: *p < 0.05, **p < 0.01, and ***p < 0.001 by one-way ANOVA followed by Tukey’s post hoc analysis.